The Varroa Mite was detected at the Port of Newcastle, prompting authorities in New South Wales to throw up a strict biosecurity zone.
Australia is said to be the only major honey-producing country where the Varroa Mite is not endemic.
To stop the spread, keepers inside the 50-kilometre (31-mile) biosecurity zone will not be able to move hives, bees, honey or comb until further notice.
The state Department of Primary Industries said the measures aimed at "ensuring we eradicate the parasite".
The tiny red-brown mites attack and feed on honey bees -- killing entire colonies, although not those of Australia's native bees.
The country's honey industry, however, relies primarily on non-native species.
"It is critically important that beekeepers in the Newcastle area do not move any hives or equipment in or out of the area," said the Australian Honey Bee Industry Council.
"Moving hives away will only further exacerbate the issue and make eradication efforts futile."
Aside from the honey industry, bees are essential in pollinating countless plants.
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